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KMID : 0352519840210030081
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Korea Univercity Medical Journal
1984 Volume.21 No. 3 p.81 ~ p.88
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Studies on the changing patterns of specifie IgG antibody in the sera of rabbits infected with Clonorchis sinensis
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梁元容/Yang, Won-Yong
李駿商/林漢鍾/Lee, Joon-Sang/Rim, Han-Jong
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Abstract
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Human clonorchiasis is one of the most common trematode diseases and of very important public health problems in Korea.
In the present study, the changing patterns of specific IgG antibody was demonstrated by the enzyme-linked immunosorbent assay(ELISA) in the sera of rabbits infected with Clonorchis sinensis.
The O.D. (optical density) values of the ELISA in the sera were measured according to intensity and course of infections and tendency of decrease after chemotherapy.
The ELISA was performed with veronal buffered saline antigen of Clonorchis, peroxidase conjugated antirabbit IgG and ortho-phenylenediamine as a substrate by micro-method. In the 3 groups of rabbits infected with 50(A group), 500(B group) or 100 x 5 (C group) metacercariae, the sera were prepared biweekly up to 18th week before chemotherapy, and biweekly up to 24th week after chemotherapy.
The results were as follows:
1. The O.D. values increased gradually with lapse of time before treatment. The O.D. values indicated firstly 1.000 or more in 8 weeks after infection in A group and 4 weeks after infection in both B and C groups.
The O.D. values reached plateau at 10 weeks after infection in A group and 8 weeks after infection in both B and C groups. Regression equations between the O.D. values of the ELI-SA and period(week) after infection among 3 groups were as follows, y=0.09x+0.319 in A group, y=0.08x+0.826 in B group and y=0.08x+0.807 in C group.
2. The O.D. values in A group which had been treated decreased gradually and became preinfection level at 24 weeks after chemotherapy.
On the contrary, the O.D. values in A group which has not been treated showed plateau. The O.D. values in both B and C groups which had been cured decreased gradually and became preinfection level at 24 weeks after chemotherapy.
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KEYWORD
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